This study is conducted to investigate the relationship between knowledge management and the organizational culture of employees of Narxoz University in 2018. The research method is applied-survey and with nature of descriptive-correlation. The statistical population of this study consists of all 4169 employees of Narxoz University. The sample size is determined using Krejcie and Morgan table and 351 is selected using simple random sampling. Sharon Lawson's Knowledge Management Questionnaire (2003) and Cameron Quinn's Organizational Culture Questionnaire (2006) were used for data collection. The validity of this questionnaire was confirmed by relevant professors and other previous researches. The reliability of the questionnaire of knowledge management and the organizational culture was reported 0.84 and 0.82 based on Cronbach's alpha. The data were analyzed using spss24 software and Pearson test. The findings showed that there is a significant relationship between knowledge management and the culture of employees of Narxoz University. There is also a significant relationship between the components of knowledge creation, knowledge acquisition, knowledge retention, and knowledge storage and knowledge distribution with the culture of employees Narxoz University. Therefore, it is suggested that the employees of the Narxoz University through training and processes to increase their ability to acquire knowledge, create knowledge, distribute knowledge, and in general manage their knowledge, thereby organizational culture to be increased.