Employment is one of the essential consequences of the expansion of economic and cultural development in society. However, there is an imbalance in the gender structure of the society of Turkey in the field of employment. In this regard, one of the most important factors for lack of balance is cultural barriers to the employment of women. Improving the employment of rural women and increasing their abilities in society are significantly important in economic development along with increased household income. In addition, increasing the knowledge and attitude of the family toward the employment of rural women plays a crucial role in the development of entrepreneurship culture in villages. Nonetheless, there are still cultural barriers such as fanaticism, prejudice, the dominance of men and lack of attention to the activity and work of women in society. According to the results of the study, the society’s attitude to women’s responsibilities, discrimination and attitude of a community toward women, the culture of patriarchy and internalization of attitude in women were among the most important cultural barriers to employment of women in Turkey, especially in rural areas.